Dr. Rathinasamy K.
Dr. Rathinasamy K.


Office Address:

Department of Biotechnolgy, National Institute of Technology Calicut, Calicut 673601, Kerala.

Contact no:

+91 495 228 6521

Email ID:


Home Address:

Faculty Apartment B-304, National Institute of Technology Calicut, Calicut 673601, Kerala.

  • Ph. D. School of Biosciences and Bioengineering Indian Institute of Technology Bombay, Mumbai.

  • M. Tech. (Biotechnology) Jadavpur University, Kolkata.

  • B. Pharm Annai J.K.K. Sampoorani Ammal College of Pharmacy, Komarapalayam The Tamil Nadu Dr. M.G.R. Medical University, chennai.

  • Educational Qualifications

    • Ph. D. School of Biosciences and Bioengineering Indian Institute of Technology Bombay, Mumbai.

    • M. Tech. (Biotechnology) Jadavpur University, Kolkata.

    • B. Pharm Annai J.K.K. Sampoorani Ammal College of Pharmacy, Komarapalayam The Tamil Nadu Dr. M.G.R. Medical University, chennai.


    1. Jomon Sebastian , Darpan Raghav, and Krishnan Rathinasamy (2022) MD simulation-based screening approach identified tolvaptan as a potential inhibitor of Eg5. Mol Divers. doi: 10.1007/s11030-022-10482-w

    2. Mahanty S, Rathinasamy K (2021) The natural anthraquinone dye purpurin exerts antibacterial activity by perturbing the FtsZ assembly. Bioorg Med Chem. 2021 Nov 15;50:116463.

    3. Mahanty S, Rathinasamy K, Suresh D (2021) Spectral Characterization of Purpurin Dye and Its Application in pH Sensing, Cell Imaging and Apoptosis Detection. J Fluoresc. 2021 Nov 3. doi: 10.1007/s10895-021-02836-8.

    4. Ashraf SM, Mahanty S, Rathinasamy K (2021) Securinine induces mitotic block in cancer cells by binding to tubulin and inhibiting microtubule assembly: A possible mechanistic basis for its anticancer activity. Life Sci. 2021 Oct 28;287:120105. doi: 10.1016/j.lfs.2021.120105.

    5. Mahanty S, Raghav D, Rathinasamy K. (2021) Vanadocene dichloride induces apoptosis in HeLa cells through depolymerization of microtubules and inhibition of Eg5.J Biol Inorg Chem. 2021 Jun;26(4):511-531.

    6. Sebastian J, Rathinasamy K. (2021) Sertaconazole induced toxicity in HeLa cells through mitotic arrest and inhibition of microtubule assembly. Naunyn Schmiedebergs Arch Pharmacol. 394 (6):1231-1249

    7. Raghav D, Mahanty S, Rathinasamy K. (2020) Characterizing the interactions of the antipsychotic drug trifluoperazine with bovine serum albumin: Probing the drug-protein and drug-drug interactions using multi-spectroscopic approaches. Spectrochim Acta A Mol Biomol Spectrosc. 226, 117584.

    8. Divya, T T, Raghav D, Rathinasamy K, and Chakkumkumarath L (2019) Multifunctional fluorescent leucomalachite green derivatives for chemodosimetric detection of Fe 3+, specific imaging of lipid droplets and intracellular pH monitoring. New Journal of Chemistry 43, 16349-16358.

    9. Sebastian J, Rathinasamy K. (2019) Benserazide Perturbs Kif15-kinesin Binding Protein Interaction with Prolonged Metaphase and Defects in Chromosomal Congression: A Study Based on in silico Modeling and Cell Culture. Mol Inform. doi: 10.1002/minf.201900035

    10. Raghav D, Mahanty S, Rathinasamy K (2019) Biochemical and toxicological investigation of karanjin, a bio-pesticide isolated from Pongamia seed oil. Pestic Biochem Physiol. 157, 108-121

    11. Sabu C, Raghav D, Jijith US, Mufeedha P, Naseef PP, Rathinasamy K, Pramod K. (2019) Bioinspired oral insulin delivery system using yeast microcapsules, Mater Sci Eng C Mater Biol Appl. 103, 109753 https://doi.org/10.1016/j.msec.2019.109753

    12. Jaleel JA, Ashraf SM, Rathinasamy K, Pramod K (2019). Carbon dot festooned and surface passivated graphene-reinforced chitosan construct for tumor-targeted delivery of TNF-α gene. Int J Biol Macromol. 127:628-636

    13. Ravikumar M, Raghav D, Rathinasamy K, Kathiravan A, and Mothi E M (2018) DNA Targeting Long-Chain Alkoxy Appended Tin(IV) Porphyrin Scaffolds: Photophysical and Antimicrobial PDT Investigations. ACS Applied Biomaterials, 1, 1705−1716

    14. Ashraf SM, Sebastian J, Rathinasamy K (2018). Zerumbone, a cyclic sesquiterpene, exerts antimitotic activity in HeLa cells through tubulin binding and exhibits synergistic activity with vinblastine and paclitaxel. Cell Prolif. e12558. doi: 10.1111/cpr.12558

    15. Mohan L, Raghav D, Ashraf SM, Sebastian J, Rathinasamy K. (2018) Indirubin, a bis-indole alkaloid binds to tubulin and exhibits antimitotic activity against HeLa cells in synergism with vinblastine. Biomed Pharmacother. 105:506-517

    16. Raghav D, Sebastian J, Rathinasamy K (2017), Biochemical and Biophysical characterization of curcumin binding to human mitotic kinesin Eg5: Insights into the inhibitory mechanism of curcumin on Eg5. Int J Biol Macromol. 109, 1189-1208.

    17. M Ashraf S, Rathinasamy K. (2017) Antibacterial and anticancer activity of the purified cashew nut shell liquid: implications in cancer chemotherapy and wound healing. Nat Prod Res., doi: 10.1080/14786419.2017.1380022.

    18. Mahanty, S., Raghav, D., and Rathinasamy, K. (2017) In vitro evaluation of the cytotoxic and bactericidal mechanism of the commonly used pesticide triphenyltin hydroxide, Chemosphere, 183, 339-352.

    19. Raghav, D. Ashraf, S.M., Mohan, L., and Rathinasamy, K. (2017) Berberine induces toxicity in HeLa cells through perturbation of microtubule polymerization by binding to tubulin at a unique site, Biochemistry 56, (20) 2594-2611

    20. Raghav, D., Maniyadath, B., Mohan, A., Sairam, S., and Rajan, R. M., and Rathinasamy, K. (2016) In-silico Designing of Novel Camptothecin Analogues as Potent Inhibitors of Topoisomerase I: A Molecular Docking, QSAR, and ADME-T Study, Letters in Drug Design & Discovery 13 (9), 859-868.

    21. Prasannan, D., Raghav, D., Sujatha, S., Rathinasamy, K., and Arunkumar, C. (2016) Synthesis, structure, photophysical, electrochemical properties and antibacterial activity of brominated BODIPYs,  RSC Advances 6 (84), 80808-80824.

    22. Soman, R., Raghav, D., Sujatha, S., Kumar, H.H., Rathinasamy, K.,  and   Chellaiah, A. (2015) Axial ligand modified high valent tin (IV) porphyrins: synthesis, structure, photophysical studies and photodynamic antimicrobial activities on Candida albicans, RSC Advances 5, 61103-61117.

    23. Appadurai, P. and Rathinasamy, K. (2015) Plumbagin-silver nanoparticle formulations enhance the cellular uptake of plumbagin and its antiproliferative activities IET Nanobiotechnol., pp. 1–9

    24. Appadurai, P. and Rathinasamy, K. (2014) Indicine N-oxide binds to tubulin at a distinct site and inhibits the assembly of microtubules: A mechanism for its cytotoxic activity, Toxicol. Lett. 225, 66-77.

    25. Parvathy R. Chandran, Prakash Appadurai, K. Rathinasamy, and N. Sandhyarani (2013)
      Enhanced Cytotoxic Effect of Doxorubicin Loaded Multifunctional Gold Nanoparticle for Targeted Cancer Drug Delivery, J. Nanopharmaceutics Drug Delivery 1, 289-300

    26. Rathinasamy, K., Jindal, B., Asthana, J., Singh, P., Balaji, P.V., and Dulal Panda (2010) Griseofulvin stabilizes microtubule dynamics, activates p53 and inhibits the proliferation of MCF-7 cells synergistically with vinblastine, BMC-Cancer 10, 213

    27. Rathinasamy, K. and Panda, D. (2008) Kinetic stabilization of microtubule dynamic instability by benomyl increases the nuclear transport of p53, Biochem. Pharmacol. 76, 1669-1680.

    28. #Clement, M. J., #Rathinasamy, K., Adjadj, E., Toma, F., Curmi, P. A., and Panda, D. (2008) Benomyl and colchicine synergistically inhibit cell proliferation and mitosis: evidence of distinct binding sites for these agents in tubulin, Biochemistry 47, 13016-13025. #Authors contributed equally

    29. Suresh, D., Balakrishna, M. S., Rathinasamy, K., Panda, D., and Mobin, S. M. (2008) Water-soluble cyclodiphosphazanes: synthesis, gold(i) metal complexes and their in vitro antitumor studies, Dalton Trans. 2812-2814

    30. Suresh, D., Balakrishna, M. S., Rathinasamy, K., Panda, D., and Mague, J. T. (2008) Large-bite bis(phosphite) ligand containing mesocyclic thioether moieties: synthesis, reactivity, group 11 (Cu(I), Au(I)) metal complexes and anticancer activity studies on a human cervical cancer (HeLa) cell line, Dalton Trans. 2285-2292

    31. Singh, P., Rathinasamy, K., Mohan, R., and Panda, D. (2008) Microtubule assembly dynamics: An attractive target for anticancer drugs, IUBMB. Life 60, 368-375

    32. Kunwar, A., Barik, A., Mishra, B., Rathinasamy, K., Pandey, R., and Priyadarsini, K. I. (2008) Quantitative cellular uptake, localization and cytotoxicity of curcumin in normal and tumor cells, Biochim. Biophys. Acta 1780, 673-679.

    33. Prasad, N. K., Rathinasamy, K., Panda, D., and Bahadur, D. (2007) Mechanism of cell death induced by magnetic hyperthermia with nanoparticles of gamma-MnxFe2-xO3 synthesized by a single step process, Journal of Materials Chemistry 17, 5042-5051. (Journal cover article).

    34. Prasad, N. K., Rathinasamy, K., Panda, D., and Bahadur, D. (2007) TC-tuned biocompatible suspension of La0.73Sr0.27MnO3 for magnetic hyperthermia, J. Biomed. Mater. Res. B Appl. Biomater. 85, 409-416.

    35. Gupta, K. K., Bharne, S. S., Rathinasamy, K., Naik, N. R., and Panda, D. (2006) Dietary antioxidant curcumin inhibits microtubule assembly through tubulin binding, FEBS J. 273, 5320-5332.

    36. Rathinasamy, K. and Panda, D. (2006) Suppression of microtubule dynamics by benomyl decreases tension across kinetochore pairs and induces apoptosis in cancer cells, FEBS J. 273, 4114-4128. (Journal cover article).

    37. Panda, D., Rathinasamy, K., Santra, M. K., and Wilson, L. (2005) Kinetic suppression of microtubule dynamic instability by griseofulvin: implications for its possible use in the treatment of cancer, Proc. Natl. Acad. Sci. U. S. A 102, 9878-9883. (Journal cover article).

    Research Interests

    •  Identification and characterization of natural compounds that exhibit selective cytotoxic activity against cancer cells and develop novel strategies in the treatment of cancer.

    • To study the role of cytoskeleton proteins in various cellular functions such as cell adhesion, migration, intracellular transport, signal transduction, mitosis and cell division.

    • To study various signal transduction pathways that are involved in cell survival and apoptosis.

    Links for the cover articles

    Recognition & Awards

    • Received Dr. Gargi Vishnoi Memorial prize for the most outstanding Ph. D Thesis in the year 2007-2008.

    • Published 3 Papers as cover articles in reputed international journals like Proc. Natl. Acad. Sci. USA 2005, FEBS Journal, 2006 and J. Mater. Chem. 2007

    • Received the award as the second best Research presentation in the 4th In- house symposium on Materials Research, (March 2007) in IIT Bombay, Mumbai.

    • Qualified the CSIR-UGC Lectureship-NET held on 1st July 2001.

    • Qualified the Combined Biotechnology Entrance Examination conducted by Jawaharlal Nehru University, New Delhi for admission to M. Tech programme in 1999.

    List of Phds produced


    Title of the Thesis Name of the Research Scholar Name of supervisors Date of award/Submis sion/Viva voce
    Cytotoxic mechanism of Indicine N-oxide and Plumbagin- loaded Silver nanoparticles: An in vitro evaluation in human cancer cell lines Prakash Appadurai Principal Guide: Dr. Rathinasamy K Defense date: 04-07-2014
    Studies on the anticancer activities of selected traditional medicinal plants with a detailed cytotoxic mechanism of Indirubin Lakshmi Mohan Principal Guide: Dr. Rathinasamy K Defense date: 04-07-2014
    Characterisation of small molecule inhibitors targeting microtubules and eg5: a detailed investigation on the anti-mitotic mechanism of berberine, curcumin, and carbamazepine Darpan Raghav Principal Guide: Dr. Rathinasamy K Defense date: 17-11-2020
    Exploration of mitotic poisons from plants: a detailed investigation on the anticancer and antimitotic activity of securinine and zerumbone Shabeeba M Ashraf Principal Guide: Dr. Rathinasamy K Defense date: 17-11-2020
    Identification and characterisation of sertaconazole and tioconazole as potential anticancer agents with microtubule depolymerising activity based on in silico and in vitro analysis Susobhan Mahanty Principal Guide: Dr. Rathinasamy K Defense date: 18-10-2021
    A Comprehensive Study On The Cell Division Inhibitory Mechanism Of The Small Molecules Triphenyltin Hydroxide, Vanadocene Dichloride And Purpurin Using Cell Culture, Biochemical And Biophysical Techniques Jomon Sebastian Principal Guide: Dr. Rathinasamy K Defense date: 20-10-2021

    Sponsored Research

    1. Faculty Research Grant (FRG10/0109), NITC, Kozhikode - Rs. 5 Lakhs.

    2. DST Project (SR/SO/BB-13/2010) granted under the SERC scheme – Rs.38,05,068.

    3. Department of Biotechnology Grant (BT/PR14142/PBD/17/696/2010) – Rs. 19.56 Lakhs.

    Invited Talks

    Delivered a talk on ?Suppression of Microtubule dynamics by benomyl increases the nuclear transport of p53? in TIFR-MotorConference 2007 held in the Tata Institute of Fundamental Research, Mumbai


    1. Awarded CSIR-Senior research Fellowship from CSIR, New Delhi from March 2007-April 2008.

    2. Received Teaching Assistanship from the Indian Institute of Technology Bombay during 2002-2006.